TY - JOUR
T1 - Non-rhinovirus enteroviruses associated with respiratory infections in Peru (2005-2010)
AU - Huaman, Jose L.
AU - Carrion, Gladys
AU - Ampuero, Julia S.
AU - Gomez, Jorge
AU - Ocaña, Victor
AU - Paz, Irmia
AU - Gomez, Elizabeth
AU - Chavez, Edward
AU - Sarmiento, Favio
AU - Pozo, Edward
AU - Laguna-Torres, V. Alberto
AU - Halsey, Eric S.
N1 - Publisher Copyright:
© 2014 Huaman et al.; licensee BioMed Central Ltd.
PY - 2014/9/22
Y1 - 2014/9/22
N2 - Background: Enteroviruses (EVs) are a common cause of respiratory tract infections and are classified into seven species (EVA-D and rhinoviruses [RHVs] A-C) with more than 200 different serotypes. Little is known about the role of non-RHV EVs in respiratory infections in South America. The aim of this study was to describe the epidemiology of non-RHV EVs detected in patients with influenza-like illness enrolled in a passive surveillance network in Peru. Methods. Throat swabs and epidemiological data were collected from participants after obtaining verbal consent. Viral isolation was performed in cell culture and identified by immunofluorescence assay. Serotype identification of EV isolates was performed using commercial monoclonal antibodies. Identification of non-serotypeable isolations was carried out by reverse transcriptase-PCR, followed by sequencing. Results: Between 2005 and 2010, 24,239 samples were analyzed, and 9,973 (41.1%) possessed at least one respiratory virus. EVs were found in 175 samples (0.7%). Our results revealed a clear predominance of EVB species, 90.9% (159/175). No EVDs were isolated. The mean and median ages of EV-positive subjects were 9.1 and 4.0 years, respectively, much younger than the population sampled, 17.6 and 12.0 years. Sixteen serotypes were identified, four EVA, 11 EVB, and one EVC species. The most common serotypes were coxsackievirus B1, coxsackievirus B2, coxsackievirus B5, and coxsackievirus B3. Conclusion: This study provides data about the serotypes of EVs circulating in Peru and sets the need for further studies.
AB - Background: Enteroviruses (EVs) are a common cause of respiratory tract infections and are classified into seven species (EVA-D and rhinoviruses [RHVs] A-C) with more than 200 different serotypes. Little is known about the role of non-RHV EVs in respiratory infections in South America. The aim of this study was to describe the epidemiology of non-RHV EVs detected in patients with influenza-like illness enrolled in a passive surveillance network in Peru. Methods. Throat swabs and epidemiological data were collected from participants after obtaining verbal consent. Viral isolation was performed in cell culture and identified by immunofluorescence assay. Serotype identification of EV isolates was performed using commercial monoclonal antibodies. Identification of non-serotypeable isolations was carried out by reverse transcriptase-PCR, followed by sequencing. Results: Between 2005 and 2010, 24,239 samples were analyzed, and 9,973 (41.1%) possessed at least one respiratory virus. EVs were found in 175 samples (0.7%). Our results revealed a clear predominance of EVB species, 90.9% (159/175). No EVDs were isolated. The mean and median ages of EV-positive subjects were 9.1 and 4.0 years, respectively, much younger than the population sampled, 17.6 and 12.0 years. Sixteen serotypes were identified, four EVA, 11 EVB, and one EVC species. The most common serotypes were coxsackievirus B1, coxsackievirus B2, coxsackievirus B5, and coxsackievirus B3. Conclusion: This study provides data about the serotypes of EVs circulating in Peru and sets the need for further studies.
KW - Enterovirus
KW - Peru
KW - Respiratory infections
UR - http://www.scopus.com/inward/record.url?scp=84907962138&partnerID=8YFLogxK
U2 - 10.1186/1743-422X-11-169
DO - 10.1186/1743-422X-11-169
M3 - Article
C2 - 25244984
AN - SCOPUS:84907962138
SN - 1743-422X
VL - 11
JO - Virology Journal
JF - Virology Journal
IS - 1
M1 - 169
ER -